HORDB

ID: hor006019
Uniprot ID: P85162
Protein name: Bradykinin-potentiating peptide 12c
Gene name: NA
Organism: Bothrops jararaca (Jararaca) (Bothrops jajaraca)
Family: Bradykinin-potentiating peptide family
Source: animal
Expression: This protein seems to be found in newborn B.jararaca venom but not in adult snake venom. |Expressed by the venom gland.
Disease: NA
Comments: NA
Taxonomy: Bothrops (genus), Crotalinae (subfamily), Viperidae (family), Colubroidea (superfamily), Serpentes (infraorder), Toxicofera , Episquamata , Unidentata , Bifurcata , Squamata (order), Lepidosauria (class), Sauria , Sauropsida , Amniota , Tetrapoda , Dipnotetrapodomorpha , Sarcopterygii (superclass), Euteleostomi , Teleostomi , Gnathostomata , Vertebrata , Craniata (subphylum), Chordata (phylum), Deuterostomia , Bilateria , Eumetazoa , Metazoa (kingdom), Opisthokonta , Eukaryota (superkingdom),cellular organisms
GO MF: GO:0004857 enzyme inhibitor activity; GO:0008191 metalloendopeptidase inhibitor activity; GO:0030414 peptidase inhibitor activity; GO:0090729 toxin activity
GO BP: GO:0008217 regulation of blood pressure; GO:0035821 modulation of process of another organism
GO CC: GO:0005576 extracellular region

Function: [Bradykinin-potentiating peptide 9a]: Inhibits the activity of the angiotensin-converting enzyme (ACE) by a preferential interaction with its C-domain. May also potentiate the hypotensive effects of bradykinin.; [Bradykinin-potentiating peptide 9a-F]: Has much lower activity than BPP-9a.
Mechanism: Bradykinin-potentiating peptide 9a-F is present only in female snakes.
Cross BBB: NA
Target: NA
Target Unid: NA
IC50: NA
EC50: NA
ED50: NA
kd: NA
Half life: NA

Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)

hor006019_AF2.pdb

hor006019_ESM.pdb

Mass:	170011	Formula:	C72H102N20O16
Absent amino acids:	CDEFGHKLMNSTVY	Common amino acids:	P
pI:	10.55	Basic residues:	1
Polar residues:	0	Hydrophobic residues:	4
Hydrophobicity:	-140.83	Boman Index:	-2015
Half-Life:	0.8 hour	Half-Life Yeast:	10 min
Half-Life E.Coli:	>10 hour	Aliphatic Index	40.83
Instability Index:	3610.83	Extinction Coefficient cystines:	11000
Absorbance 280nm:	1000

PubMed ID: 15245866
Title: Identification of five new bradykinin potentiating peptides (BPPs) from Bothrops jararaca crude venom by using electrospray ionization tandem mass spectrometry after a two-step liquid chromatography.
PubMed ID: 22869554
Title: Peptidomics of three Bothrops snake venoms: insights into the molecular diversification of proteomes and peptidomes.
PubMed ID: 20146532
Title: Analysis of the ontogenetic variation in the venom proteome/peptidome of Bothrops jararaca reveals different strategies to deal with prey.
PubMed ID: 4334402
Title: Angiotensin-converting enzyme inhibitors from the venom of Bothrops jararaca. Isolation, elucidation of structure, and synthesis.
PubMed ID: 15912471
Title: Fast analysis of low molecular mass compounds present in snake venom: identification of ten new pyroglutamate-containing peptides.
PubMed ID: 17315274
Title: Mass spectrometric analysis of the individual variability of Bothrops jararaca venom peptide fraction. Evidence for sex-based variation among the bradykinin-potentiating peptides.
PubMed ID: 11994001
Title: Selective inhibition of the C-domain of angiotensin I converting enzyme by bradykinin potentiating peptides.
PubMed ID: 200262
Title: Design of potent competitive inhibitors of angiotensin-converting enzyme. Carboxyalkanoyl and mercaptoalkanoyl amino acids.